GERMANY

Reinhard Sterner

University of Regensburg, Institute of Biophysics and physical Biochemistry, D-93040 Regensburg, Germany

 

INPEC node since 2004

 

In the focus of our research are the evolution and the design of enzymes, which are elaborate proteins that catalyze cellular reactions with high specificity and efficiency.  We are using rational design and directed evolution to engineer the stability and catalytic activity of enzymes, experimentally reconstruct the natural evolution of enzymes, assign functions to uncharacterized enzymes, and analyze allosteric interactions within multi-enzyme complexes. To this end, we apply a broad range of experimental and computational methods.

Keywords: enzyme evolution, enzyme design, functional annotation, multi-enzyme complex, allostery, ancestral sequence reconstruction, protein stability, rational design, directed evolution

Birte Höcker

Universität Bayreuth

Yves Muller

Friedrich Alexander University of Erlangen-Nürnberg